How it works:
1. Input two PDB structures representing different conformational states of the same protein.
2. The tool aligns sequences and generates all possible single-point mutations.
3. ProteinMPNN evaluates each variant for both conformational states.
4. Results show which mutations bias the protein toward one conformation or the other.
Default example: LplA (3A7R vs 1X2G) - the original case from the paper.
State 1 Configuration
4-letter = RCSB PDB; longer = AlphaFold DB
State 2 Configuration
4-letter = RCSB PDB; longer = AlphaFold DB
Leave empty to automatically compute consensus sequence from both structures.
Starting...
Summary
Result Plot
All Variants Results
Mutation ▾
State1 Score ▾
State2 Score ▾
State1 Scaled ▾
State2 Scaled ▾
S1 Bias ▾
S2 Bias ▾
Class ▾
Download full results as CSV with all scored variants and classifications.